Journal: Journal of Extracellular Vesicles

Article Title: Hyaluronic Acid Decoration Facilitates CD44‐Mediated Targeting and Alters Protein Corona Formation of Extracellular Vesicles

doi: 10.1002/jev2.70263

Figure Lengend Snippet: Label‐free MP‐SPR quantification of MCF7‐EV and HAS3‐EV interactions with CD44 + and CD44 − cells. (A) Representative SPR curve comparison between blank gold sensor, fibronectin coated sensor, CD44 + monolayer, and CD44 − monolayer. Phase contrast microscopy images of the (B) CD44 + and (E) CD44 − cell monolayers before and after the SPR measurement. Scale bars correspond to 200 µm. MP‐SPR signal of the interaction between MCF7‐EVs and (C) CD44 + cells or (F) CD44 − cells. Interaction between HAS3‐EVs with (D) CD44 + cells or (G) CD44 − cells. SPR‐curves with standard deviation from two (HAS3‐EVs with CD44 + cells) to three biological replicate measurements are shown. EV, extracellular vesicle; HA, hyaluronic acid.

Article Snippet: The SPR reflectance spectrum was recorded between 58° and 77° with a scanning time of approximately 2.0 s. Data were exported in ASCII format with MP‐SPR Navi Data Viewer 6.7.0.1 software (BioNavis), and SPR peak angular positions were determined using the weighted centroid method.

Techniques: Comparison, Microscopy, Standard Deviation

Journal: Journal of Extracellular Vesicles

Article Title: Hyaluronic Acid Decoration Facilitates CD44‐Mediated Targeting and Alters Protein Corona Formation of Extracellular Vesicles

doi: 10.1002/jev2.70263

Figure Lengend Snippet: MP‐SPR based CD9‐antibody capture of EVs and plasma‐derived corona formation. SPR responses with 670 nm (line) and 785 nm lasers (dashed line) and the ratio of the two wavelengths (dots) from CD9‐antibody captured MCF7‐EVs (A) and HAS3‐EVs (B) over 30‐min injections of EVs ( n = 3, STDEV). After EV capture, EV‐depleted plasma was injected to the system, causing a sharp increase in the SPR‐signal, followed by sharp decrease after washing with PBS (indicated with arrows). The corona refractive index and thickness were calculated from the remaining tail signal. SPR responses with 670 nm (line) and 785 nm lasers (dashed line) for plasma injection and protein corona formation on MCF7‐EVs (C) and HAS3‐EVs (D) ( n = 3, STDEV). The average ratio of the signals measured at 670 and 785 nm for the dual‐wavelength method to calculate the EV diameters, and refractive index (RI) and thickness of the protein corona determined with LayerSolver are shown in the table. EV, extracellular vesicle; HA, hyaluronic acid.

Article Snippet: The SPR reflectance spectrum was recorded between 58° and 77° with a scanning time of approximately 2.0 s. Data were exported in ASCII format with MP‐SPR Navi Data Viewer 6.7.0.1 software (BioNavis), and SPR peak angular positions were determined using the weighted centroid method.

Techniques: Clinical Proteomics, Derivative Assay, Injection, Refractive Index